Brighton Integrative Genomics (BIG) Unit

The new state-of-the-art, custom designed Brighton Integrative Genomics (BIG) Unit is housed within the Moulsecoomb campus at the University of Brighton.

The facility was established with significant grants and investment from the Natural Environment Research Council (NERC), Biotechnology and Biological Sciences Research Council (BBSRC), the European Commission, Higher Education Funding Council for England (HEFCE) and the University of Brighton.

The equipment, expertise and services listed below are accessible to academics, individuals and industry.

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The Brighton Integrative Genomics Unit at the University of Brighton

The Brighton Integrative Genomics Unit is a self contained facility run Professor of Genomics and Precision Cardiovascular Medicine and Director of Brighton Integrative Genomics Unitby Director, Rameen Shakur, with Research Fellow, Dr Giselda Bucca and Bioinformatics Experimental Officer, Dr Andrew Hesketh.

If you are interested in accessing any of the services detailed below, you can contact the team directly at Genomics@brighton.ac.uk or by completing the enquiry form, accessible via the link in the red box in the top right above.

Sequencing

Next Generation Sequencer (NGS): Illumina NextSeq500 sequencer

High Output kit (up to 400 million clusters)	Single end (custom, 75, 150)	Paired end (custom, 75, 150 bp)
Medium Output kit (up to 130 million clusters)	Single end (custom, 150 bp)	Paired end (custom, 75, 150 bp)

Library preparation

Quality control of nucleic acids:

Labtech Nanodrop One C spectrophotometer
Agilent 4200 TapeStation
Qubit 2.0 fluorometer
AriaMx qPCR machine
Alpha cycler 4 PCR machine

DNA size selection:

Blue Pippin (Sage Science)
Covaris M220 focussed DNA ultra-sonicator

Major equipment for nucleic acid QC and quantification

Agilent 4200 TapeStation System

Instrument for the quality control of nucleic acids: integrity (RIN-equivalent scoring), sizing and approximate quantification.

Agilent 4200 Tapestation for QC

Quantitative real time PCR

Agilent AriaMx Real time PCR system for accurate library quantification.

Agilent AriaMx qPCR machine

Applications supported at Brighton Genomics

DNA sequencing

De novo genome sequencing (bacterial, small genomes and large genomes)
Exome sequencing
Amplicon sequencing
ChIP sequencing
Methylome sequencing (collaborative projects only)

DNA microarrays

Expression microarrays (including RNA isolation, RNA labelling, microarray hybridization, washing and scanning)

RNA-seq

Small RNA sequencing
Total RNA sequencing
Stranded mRNA sequencing

Ribosome profiling

Bacterial and eukaryotic systems (collaborative projects only)

Prices for sequencing only*

*(quality controlled normalised libraries to be provided by user). See Sample Preparation Guidelines (pdf).

To obtain specific pricing for preparation of samples and sequencing libraries for any of the above applications, please complete the enquiry form.

Using NextSeq 500 sequencing kits:

75 cycles up to 400 million clusters	1 x 75 bp (or less); 2 x 33 bp PE	£1,557
150 cycles up to 400 million clusters	1 x 150 bp; 2 x 75 bp PE	£2,713
300 cycles up to 400 million clusters	2 x 150 bp PE	£4,153
150 cycles up to 130 million clusters	1 x 150 bp; 2 x 75 bp PE	£1,182
300 cycles up to 130 million clusters	2 x 150 bp PE	£1,765

Equipment for hire on site

Name	Manufacturer	Description
Next Seq 500	Illumina	Next Generation Sequencer
Microarray Scanner C	Agilent Technologies	DNA microarray scanner at 2 μm resolution
Oven for DNA microarray hybridization	Agilent Technologies	Oven with rotisserie for the hybridization of DNA microarrays
AriaMx	Agilent Technologies	Real time PCR equipment
4200 TapeStation	Agilent Technologies	Instrument for the quality control of nucleic acids
Nanodrop One C	LabTech International	Spectrophotometer for small volumes
PCR alpha cycler 4	LabTech	PCR multiblock
Vilber Lourmat Fusion FX Integrated imaging system	LabTech	Digital gel imaging system for detecting fluorescent, chemiluminescence and infrared signals
Tissuelyser	Qiagen	Cell disruption system
Blue Pippin	Sage Science	DNA size fractionation system
M220 Focussed ultra-sonicator	Covaris	Instrument for the controlled shearing of DNA for NGS
Qubit 2.0 Fluorometer	Thermo Fisher Scientific	Fluorometer for nucleic acid quantification

Additional equipment

Name	Manufacturer	Description
Eppendorf refrigerated centrifuge 5430R+rotor FA-4	Thermo Fisher Scientific	Centrifuge for 1.5ml tubes
Eppendorf Thermomixer C	Thermo Fisher Scientific	Mixer/incubator for 1.5ml/0.2ml tubes
Eppendorf Concentrator F-45-48-11 GB	Thermo Fisher Scientific	Vacuum concentrator
Labcaire chemical fume hood Aura 250/255 and 2x AC safety filters	Thermo Fisher Scientific	Chemical fume hood
Haier Upright Freezer, 728 litre capacity	Scientific Laboratory Supplies	-80°C freezer
Ice maker	Hoshizaki	Ice maker
Water purification system	ELGA	Water purification system up to 18.2 megohms grade