NAD(P)H quinone oxidoreductase 1 (NQO1; DT-diaphorase) is a broad specificity enzyme which reduces a range of substrate including quinones. We are seeking to understand how this protein works and how variant forms of this predispose people to cancer. Our studies focus on the recombinant human enzyme and combine biochemical, enzymological, biophysical and in silico work. However, we also aspire to generate yeast “models” to enable us to study variants under controlled in vivo conditions and we are open to collaborations from colleagues who are studying the enzyme in cell culture or other in vivo systems. In related work, we are also interested in the related enzyme, NRH quinone oxidoreductase 2 (NQO2), the yeast quinone oxidoreductase Lot6p and the bacterial enzyme MdaB.
This project commenced in 2006 and will end when we have addressed all the interesting questions.
Dr Angel Pey, University of Grenada
Prof Ian Stratford, University of Manchester
Dr Richard Bryce, University of Manchester
Dr Clare Megarity, University of Oxford